The lentiviral vector for gene knockdown allows for the direct cloning of shRNA oligos using alpha-complementation, and for the monitoring of induction of RNA
The vector is engineered to be Tet-On and of doxycycline
Because many different doxycycline-inducible lentiviral vectors have been described
In this study, we investigated the role of KLF4 in ovarian cancer cells by transducing the ovarian cancer cell lines SKOV3 and OVCAR3 with a doxycycline-inducible KLF4 lentiviral vector
Specifically, the power of inducible lentiviral tools in vivo has been limited by inconsistent inducibility in various cell types and across polyclonal populations
For selecting the best adipogenic lentiviral expression backbone, the P aP2 or P′ aP2 driving inducible LucGFP lentiviral expression vectors were transfected into the plated cells with or without the co lentiviral particles
The V10 variant performed optimally in the transiently transfected cells and Doxycycline-inducible lentiviral expression of SpCas9 Depositing Labs
doi: 10
Characterization of an immune-evading doxycycline-inducible lentiviral vector for gene therapy in the spinal cord Exp Neurol
2022
Left: mESCs 2 d after EGFP infection; scale The prototype tetracycline (Tc) and derivatives like doxycycline (dox) have been widely used in humans as antibiotic
We are proud to offer two different IPTG inducible vectors for your research